Protocolo para diagnóstico y tratamiento de sarcomas de tejidos blandos del hospital de Basurto / Protocol for diagnosis and treatment of soft tissue sarcomas at the hospital of Basurto

La preservación de la función en el tratamiento de los sarcomas de tejidos blandos (STB) se consigue por medio de resecciones marginales, reconstrucción de tejidos blandos y terapia adyuvante sin que ello implique un aumento en las tasas de recidiva local, de metástasis o de mortalidad; exigen un abordaje multidisciplinario por lo que es necesario coordinar, bajo la forma de Comisión, a los diferentes especialistas que intervienen en las fases diagnóstica y terapéutica. Este es un artículo de revisión del diagnóstico y tratamiento de los sarcomas de tejidos blandos con motivo de la presentación del protocolo establecido en el Hospital de Basurto (Bilbao, España), vigente desde febrero de 2001 que intenta asegurar que los pacientes se beneficien de un tratamiento óptimo (AU)

The preservation of the function in the treatment of Soft Tissue Sarcomas (STS) has been achieved by means of marginal resections, reconstruction of soft tissues and adjuvant therapy without it implies an increase in the rates of local relapse, metastasis or mortality. It demands a multidisciplinary orientation for what is necessary to coordinate, under the form of Commission, the different specialists that take part in the diagnostic and therapeutic phases. This is a review article of the diagnosis and treatment of soft tissues sarcomas because of the protocol presentation settled down in the Hospital of Basurto (Bilbao, España), effective from February of 2001, that tries to assure patient’s benefit with optimal care (AU)

Informe de la actividad del comité de tumores de partes blandas del hospital de Basurto / Report of the activity of the soft tissue tumors committee of the hospital of Basurto

La captación, registro y seguimiento de los pacientes que padecen un tumor de partes blandas es un proceso necesario para asegurar que el paciente es tratado, para coordinar, de una forma completa y sin demoras las fases diagnóstica y terapéutica, y para valorar los resultados de los protocolos aplicados. Entre febrero de 2001 y julio de 2002 fueron presentados 106 pacientes en el Comité de Tumores de Partes Blandas (CTPB) del Hospital de Basurto (Bilbao, España). En esta publicación hacemos un informe descriptivo de la actividad de nuestro Comité y de su funcionamiento (AU)

The reception, registration and follow-up of patients that suffer from a soft tissue tumour are a necessary process to assure adecuate treatment of those patients, to coordinate, in a comprehensive way and without delays, the diagnostic and therapeutic steps and to evaluate the results of the applied protocols. Between February 2001 and July 2002, 106 patients were visited at the Soft Tissue Tumours Committee of the Hospital de Basurto (Bilbao, Espa- ña). In this publication we make a description of the patients included in our protocol and a comment on the way of working of the Committee (AU)

Sex-dependent synaptic behaviour in triploid turbot, Scophthalmus maximus (Pisces, Scophthalmidae).

A surface-spreading synaptonemal complex (SC) technique was used to analyse spermatocytes and oocytes of triploid turbot (Scophthalmus maximus) in order to visualise the process of chromosome synapsis. The most conspicuous characteristic of triploid oocytes is that, in the trivalents, the lateral elements of the SC were frequently associated in threes, either completely along the length of the trivalent, or partially, forming a variety of forked structures. In these nuclei, synapsis usually occurred among homologous chromosomes and the number of bivalents observed was significantly higher than that expected under the assumption of random chromosome association among all partners. However, the frequency of trivalents was very low in triploid spermatocytes, triple synapsis being also scarce. In these nuclei chromosomes that were excluded from homologous synapsis become engaged in random SC formation, and, therefore a considerable number of non-homologous associations are produced. The causes of the synaptic differences observed in triploid males and females of turbot and their possible relation to the sterility displayed by these animals are discussed.

Synaptonemal complex analysis in spermatocytes and oocytes of turbot, Scophthalmus maximus (Pisces, Scophthalmidae).

A surface-spreading synaptonemal complex (SC) technique was used to analyze spermatocytes and oocytes of turbot (Scophthalmus maximus) to visualize the process of chromosome synapsis. The total SC length was 205 +/- 12 microm in males and 172 +/- 29 microm in the only female analyzed. A representative SC karyotype of turbot was obtained. Each SC showed lateral elements of equal length. No bivalent exhibiting atypical synaptic behaviour that could be associated with heteromorphic sex chromosomes was observed, either in males or in the female. The DNA content of turbot was evaluated in eight individuals of both sexes by flow cytometry analysis. The 2C mean DNA content of turbot (1.308 +/- 0.009 pg/cell) was among the lowest observed within fishes. No statistical differences in DNA content were revealed between the sexes [Wilcoxon/Mann-Whitney test; P(W(x) = 0.243)]. The SC/DNA content ratio observed in turbot was the highest reported to date in bony fishes (Osteichthyes).